The Significance and Detection of VBNC Microorganisms
Microbiologists have long recognized there are significant numbers of microorganisms in the environment that cannot be cultured successfully with conventional methods. A wide range of nonsporulating gram-negative and gram-positive bacteria can exist in the Viable But Non Culturable state.
Analytical methods will need to change if a true assessment of the significance of such organisms is to be made, and there is evidence that this is happening.
This paper/webinar discusses and compares many detection methods including:
- Direct counting methods
- Dye reduction tests
- Immunological-based methods
- Genetic methods
- ATP bioluminescence
- Fluorescent labeling methods
- Optical fluorescence