The Significance and Detection of VBNC Microorganisms

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Microbiologists have long recognized there are significant numbers of microorganisms in the environment that cannot be cultured successfully with conventional methods. A wide range of nonsporulating gram-negative and gram-positive bacteria can exist in the Viable But Non Culturable state.

Analytical methods will need to change if a true assessment of the significance of such organisms is to be made, and there is evidence that this is happening.

This paper/webinar discusses and compares many detection methods including:

  • Direct counting methods
  • Dye reduction tests
  • Immunological-based methods
  • Genetic methods
  • ATP bioluminescence
  • Fluorescent labeling methods
  • Optical fluorescence